We are very much looking forward to a visit from Dr. Fillip Port from the Laboratory of Molecular Biology at the MRC in Cambridge. Leading the CRISPR project in the lab of Simon Bullock, Fillip has pioneered the use of the Cas9 endonuclease in genomic engineering of Drosophila. This technique now allows the introduction of mutations at single nucleotide resolution and to integrate exogenous sequences at predefined loci. Today a Drosophila toolbox is available that consists of transgenic cas9 strains and versatile gRNA expression plasmids for various applications, including germ line mutagenesis, precise knock-ins and tissue specific genetic screens. Further information about this can be found on the CRISPR fly design webpage and in the Blog.
In his talk Fillip will present how he is using CRISPR/cas-mediated genome engineering to gain novel insights into the mechanism and functional relevance of mRNA localisation in the nervous system. Feel free to join us for some exciting science on November 3rd at 5p.m. in H53!